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KMID : 0606920180260020146
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Biomolecules & Therapeutics
2018 Volume.26 No. 2 p.146 ~ p.156
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Spermidine Protects against Oxidative Stress in Inflammation Models Using Macrophages and Zebrafish
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Jeong Jin-Woo
Cha Hee-Jae
Han Min-Ho
Hwang Su-Jung
Lee Dae-Sung
Yoo Jong-Su
Choi Il-Whan
Kim Suhk-Mann
Kim Heui-Soo
Kim Gi-Young
Hong Su-Hyun
Park Cheol
Lee Hyo-Jong
Choi Yung-Hyun
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Abstract
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Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), and cytokines including tumor necrosis factor-α and interleukin-1β in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects of spermidine accompanied by a marked suppression in their regulatory gene expression at the transcription levels. Spermidine also attenuated the nuclear translocation of NF-κB p65 subunit and reduced LPS-induced intracellular accumulation of reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, spermidine prevented the LPS-induced NO production and ROS accumulation in zebrafish larvae and was found to be associated with a diminished recruitment of neutrophils and macrophages. Although more work is needed to fully understand the critical role of spermidine on the inhibition of inflammation-associated migration of immune cells, our findings clearly demonstrate that spermidine may be a potential therapeutic intervention for the treatment of inflammatory and oxidative disorders.
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KEYWORD
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Spermidine, Macrophages, Zebrafish, Anti-Inflammation, Anti-Oxidant
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